Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Jennifer Hale

Felicia Hardy (voice)

Linda Gary

Aunt May Parker (voice)

Gary Imhoff

Harry Osborn (voice)

Ed Asner

J. Jonah Jameson (voice)

Rodney Saulsberry

Joe "Robbie" Robertson (voice)

Roscoe Lee Browne

Kingpin (voice)

Joseph Campanella

Dr. Curt Connors (voice),Dr. Curt Connors / Lizard (voice),Lizard (voice)

Nick Jameson

Michael Morbius (voice),Richard Fisk (voice),Morbius (voice),.....