Drake Bell

Peter Parker / Spider-Man (voice)

Misty Lee

Aunt May Parker (voice)

J.K. Simmons

J. Jonah Jameson (voice)

Ogie Banks

Luke Cage / Power Man (voice)

Chi McBride

Nick Fury (voice)

Clancy Brown

,Taskmaster (voice)

Logan Miller

Sam Alexander / Nova (voice)

Matt Lanter

Flash Thompson / Agent Venon (voice),Harry Osborn (voice),Flash Thompson / Agent Venom / Harry Osborn (voice)

Travis Willingham

Skurge (voice),,Thor (voice)

Kevin Michael Richardson

Groot (voice),