Benjamin Valic

Peter Parker / Spider-Man (Spidey) (voice)

Lily Sanfelippo

Gwen Stacy / Ghost-Spider (voice)

Jakari Fraser

Miles Morales / Spider-Man (Spin) (voice)

JP Karliak

Norman Osborn / Green Goblin (voice)

Kelly Ohanian

Carolyn Trainer / Doctor Octopus (voice),Carolyn Trainer / Dr. Octopus (voice)

Melanie Minichino

Aunt May (voice)

Justin Shenkarow

Aleksei Sytsevich / Rhino (voice)

Stephanie Lemelin

Francine Frye / Electro (voice)

Armen Taylor

Bruce Banner / Hulk (voice)

Dee Bradley Baker

Trace-E (voice),Trace-E / CAL (voice)