Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney

Andrew Glouberman (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Andrew Rannells

Matthew MacDell (voice),

Jenny Slate

Missy Foreman-Greenwald (voice)

Ayo Edebiri

Missy Foreman-Greenwald (voice),Missy Foreman-Greenwald / Mosaic Missy (voice)

Joe Wengert

Caleb (voice),Caleb / Lump Humpman (voice),Teacher (voice),.....